Journal: bioRxiv

Article Title: Mobius Assembly: a versatile framework for Golden Gate assembly

doi: 10.1101/140095

Figure Lengend Snippet: (A) A schematic showing the four intermediate Level 2 constructs for the assembly of the 16-TU construct. The carotenoid biosynthesis genes crtE, crtB, crtI , and crtY assembled in the Vector A, the yellow chromoprotein genes scOrange, amilGFP, amajLime , and fwYellow in the Vector B, the pink chromoprotein genes tsPurple, eforRed, spisPink , and mRFP1 in the Vector Γ, and the violacein biosynthesis genes vioA, vioB, vioD and vioE in the Vector Δ. (B) A schematic of the 16TU construct derived from the assembly of the four Level 2 cassettes, each containing 4-TUs, in the Level 1 Acceptor Vector A. (C) Cells transformed with the successfully assembled 16TU construct grew into black colonies due to predominant colouring by protoviolaceinic acid. (D) Gel electrophoresis of six plasmids (isolated from the black colonies) digested with Pst l and EcoR l resulting in bands of expected sizes - 18.3kb for the insert and 2.2kb for the vector. (E) The same plasmids were digested with Pst l and Ale l resulting in the bands of expected sizes - 7.1kb, 5.3 and 4.9kb (appear merged on the gel), and 3.2kb.

Article Snippet: Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoR l HF and Pstl-HF (NEB) for 20min at 37°C followed by purification with PCR Clean-up kit (Macherey Nagel).

Techniques: Construct, Plasmid Preparation, Derivative Assay, Transformation Assay, Nucleic Acid Electrophoresis, Isolation

Journal: iScience

Article Title: Icaritin inhibits CDK2 expression and activity to interfere with tumor progression

doi: 10.1016/j.isci.2022.104991

Figure Lengend Snippet:

Article Snippet: BamH I EcoR I , NEB , #R3136V #R3101V.

Techniques: Recombinant, Control